Thermo Fisher Scientific Occludin Recombinant Mouse Monoclonal Antibody (OC-3F10), Alexa Fluor Plus 594
Occludin 단백질을 표적하는 Alexa Fluor Plus 594 형광 표지 마우스 단일클론 항체로, ICC/IF 및 유세포분석에 최적화됨. 높은 신호 대비와 로트 간 일관성을 제공하며, 590/618 nm 형광 특성으로 밝은 시각화 가능. 연구용으로만 사용.
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Thermo Fisher Scientific Occludin Recombinant Mouse Monoclonal Antibody (OC-3F10), Alexa Fluor Plus 594
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:1,000 |
| Flow Cytometry (Flow) | 0.063–0.25 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | OC-3F10 |
| Immunogen | GST fusion protein consisting of the C-terminal region (~150aa) of human occludin |
| Conjugate | Alexa Fluor™ Plus 594 |
| Excitation / Emission Max | 590 / 618 nm |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | Proprietary buffer, pH 6.5 |
| Contains | 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Wet ice |
| RRID | AB_3093160 |
Additional Formats
- Unconjugated
- Alexa Fluor Plus 488
- Alexa Fluor Plus 647
- Custom conjugation available upon request
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies utilize proprietary dye chemistry for enhanced brightness and improved signal-to-noise ratio compared to conventional Alexa Fluor conjugates. These recombinant antibodies offer superior specificity and lot-to-lot consistency by expressing cloned antibody genes in vitro.
Usage Note:
Before use, centrifuge the conjugate solution briefly and use only the supernatant to minimize nonspecific background staining caused by aggregates.
Tested Applications:
This OC-3F10 antibody has been validated for immunocytochemistry and flow cytometry using Caco-2 cells.
- ICC: 1 µg/mL
- Flow Cytometry: ≤ 0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
Optimal dilution should be determined experimentally.
Optical Properties:
- Excitation: 590 nm
- Emission: 618 nm
- Recommended Laser: Yellow Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Occludin is a 65 kDa protein that can appear in multiple phosphorylated forms up to ~82 kDa. It plays a key role in tight junction integrity and permeability regulation. Polyunsaturated fatty acids up-regulate occludin expression, enhancing cell resistance and decreasing permeability. Expression levels vary by tissue, with high and continuous expression in brain endothelial cells. Reduced occludin expression is associated with increased tumor endothelial permeability and brain tumor edema.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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