Thermo Fisher Scientific Collagen Pan Polyclonal Antibody
Human collagen I–V 검출용 Rabbit Polyclonal Antibody로, IHC, ICC/IF, ELISA 등 다양한 응용에 적합. 비결합형 동결건조 형태로 제공되며, 높은 특이성과 낮은 교차반응성을 보유. 연구용으로 콜라겐 구조 및 질환 관련 연구에 활용 가능.
- 카탈로그번호
- PA185324
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Collagen Pan Polyclonal Antibody
Thermo Fisher Scientific Collagen Pan Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:10–1:40 | - |
| Immunocytochemistry (ICC/IF) | 1:10–1:40 | - |
| ELISA | 1:2,000–1:8,000 | - |
Product Specifications
| Property | Description |
|---|---|
| Published species | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mixture of human collagen types I–V from human placenta |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1 mg/mL |
| Storage conditions | Store at 4°C short term; for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping conditions | Wet ice |
| RRID | AB_930067 |
Product Specific Information
Reconstitute with 0.5 mL distilled water.
PA1-85324 detects Collagen I, II, III, IV, and V in human samples.
Reactivity with collagens I and II is ~70% as strong as that with III–V.
Reactivity with human fibronectin, albumin, and immunoglobulins is <0.5%.
Rabbit anti-human collagen I/II/III/IV/V antibody recognizes multiple collagen types and shows the following reactivities in ELISA with both native and denatured human collagen types:
- I & III: 100%
- IV & V: 90%
- II: 80%
- Fibronectin, albumin, and immunoglobulins: <0.5%
Target Information
Collagens are highly conserved and characterized by an uninterrupted Glycine-X-Y triplet repeat essential for triple helical structure.
Generating type-specific antibodies to collagens is challenging due to their conserved nature.
Type-specific antibodies depend on non-denatured three-dimensional epitopes, allowing preservation of native protein conformation.
Type I collagen (95 kDa) is found in bone, cornea, skin, and tendon.
Mutations in the collagen 1 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, and idiopathic osteoporosis.
Chromosomal translocations involving chromosomes 17 and 22 can lead to dermatofibrosarcoma protuberans via unregulated PDGF expression.
Two transcripts are known due to alternate polyadenylation signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 원본은 본문 하단에 위치합니다)
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.