Thermo Fisher Scientific CRYAB Recombinant Rabbit Monoclonal Antibody (JA50-32)
CRYAB 단백질을 인식하는 토끼 유래 재조합 단클론 항체로, 높은 특이성과 재현성을 제공합니다. Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합합니다. 단백질 A 정제, 1 mg/mL 농도, 장기 보관 시 -20°C에서 안정적입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Immunoprecipitation (IP) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JA50-32 |
| Immunogen | Synthetic peptide within Human Alpha B Crystallin (aa 126–175) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809975 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, followed by screening of individual clones to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the large rabbit immune repertoire
Target Information
Lens proteins consist predominantly of crystallins (about 95%), which are also found in vertebrate skeletal muscle tissue. In the lens, crystallins maintain the proper refractive index.
The mammalian lens contains three major classes of crystallins: alpha, beta, and gamma.
- Alpha-crystallin: Largest group, composed of alpha-A and alpha-B gene products, constituting ~40% of total lens protein.
- Function: Maintains refractive index and acts as a chaperone to prevent protein aggregation that could lead to cataract formation.
- Alpha-B crystallin (CRYAB): Associated with Alexander’s disease, where it accumulates in brain cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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