Thermo Fisher Scientific Periostin Recombinant Rabbit Monoclonal Antibody (JA63-40)
Recombinant rabbit monoclonal antibody (clone JA63-40) specific for human Periostin. Validated for WB and IHC-P. Offers high specificity, lot-to-lot consistency, and animal-free formulation. Supplied as liquid, 1 mg/mL, Protein A purified, stored at 4°...
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:400 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JA63-40 |
| Immunogen | Synthetic peptide within Human Periostin (aa 11–60) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809978 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and expressed in HEK293 cells. Individual clones are screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
Periostin (PN), also known as osteoblast-specific factor 2 (OSF-2), is a disulfide-linked, secreted protein that binds heparin and functions as a ligand for αVβ3 and αVβ5 integrins. It acts as a cell adhesion molecule in preosteoblasts and plays a role in osteoblast recruitment, spreading, and attachment.
Periostin is mainly detected in the lower gastrointestinal tract, aorta, stomach, placenta, uterus, and breast tissues, but is up-regulated in epithelial ovarian tumors and overexpressed in breast cancer. Its expression is induced by BMP2 and TGF-β1. The protein contains a signal sequence, a cysteine-rich domain, four fasciclin-like repeats, and a C-terminal domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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