Thermo Fisher Scientific ULK1 Recombinant Rabbit Monoclonal Antibody (JA58-36)
ULK1 단백질을 표적하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF에 적합합니다. 높은 특이성과 재현성을 제공하며, HEK293 세포에서 발현된 단백질 A 정제 항체입니다. 자가포식 연구에 유용한 연구용 시약입니다.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific ULK1 Recombinant Rabbit Monoclonal Antibody (JA58-36)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JA58-36 |
| Immunogen | Synthetic peptide within Human ULK1 (aa 930–979) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809976 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
ULK1 is a serine/threonine protein kinase essential in the initiation of autophagy, a critical cellular response to nutrient starvation.
The conserved C-terminal domain (CTD) regulates ULK1 localization and function. Knockdown of ULK1 inhibits both general and rapamycin-induced autophagy, indicating its role downstream of mTOR.
ULK1 forms a complex with FIP200 and ATG13, which are required for proper localization and stability of ULK1 during autophagosome formation. ULK1 is phosphorylated by the mTOR pathway in a nutrient-dependent manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.