Thermo Fisher Scientific Ep-CAM/CD326 (Extracellular Domain) (Epithelial Marker) Monoclonal Antibody (EGP40/1798)
Ep-CAM/CD326 단백질의 세포외 도메인을 인식하는 단클론 항체로, 인간, 개, 고양이 시료에 반응합니다. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합하며, 단백질 A/G로 정제된 액상 형태입니다. 4°C에서 보관하며 동결 금지.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 0.5–2 µg/mL | |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL | |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells | |
| ELISA | 2–4 µg/mL | |
| Peptide Array | Assay-dependent | |
| Immunomicroscopy (IM) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Dog, Cat, Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | EGP40/1798 |
| Immunogen | Recombinant fragment from the extracellular domain of human EpCAM protein (around aa100–224) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in epithelial tissues (~40 kDa). It functions as a calcium-independent cell adhesion molecule and influences cell cycle, proliferation, and metabolism by inducing proto-oncogene c-myc and cell cycle genes cyclin A and E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression is linked to enhanced epithelial proliferation and is commonly seen in human carcinomas, serving as a marker for epithelial-origin tumors.
Ep-CAM is localized on the basolateral surface of most simple epithelia and many carcinoma types, and can distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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