Thermo Fisher Scientific CCR5 Recombinant Mouse Monoclonal Antibody (RC-10)
CCR5 단백질을 특이적으로 인식하는 Mouse 단클론 항체로, Western blot, ICC/IF, ELISA 등에 사용 가능. HEK293 세포에서 발현된 recombinant 항체이며, Protein A로 정제됨. HIV 연구 및 CCR5 관련 면역학 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RC-10 |
| Immunogen | This antibody was raised by immunizing mice with CCR5. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_3074737 |
Product Specific Information
This antibody recognizes non-activated, nonphospho-CCR5.
CCR5 is a receptor for several inflammatory CC-chemokines including CCL3 (MIP-1-alpha), CCL4 (MIP-1-beta), and RANTES.
Target Information
Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells.
Among these, CCR5 (CC-CKR-5) functions as a principal coreceptor for macrophage- and dual-tropic HIV-1 strains, facilitating fusion and entry into human white blood cells.
CCR5 is essential for infection by HIV-1, HIV-2, and SIV.
The beta-chemokines RANTES, MIP-alpha, and MIP-beta serve as ligands for CCR5 and can prevent infection by M-tropic HIV-1.
CCR5 associates with the surface CD4-gp120 of the HIV complex, leading to membrane fusion and viral entry.
The amino-terminal domain and extracellular loops of CCR5 act as HIV binding sites.
CCR5 mRNA is expressed in lymphoid organs and monocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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