Thermo Fisher Scientific CCR5 Chimeric Recombinant Rabbit Monoclonal Antibody (RC-10)
CCR5 단백질을 인식하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체로, 인간 시료에 반응합니다. Western blot, ICC/IF, Flow cytometry, ELISA 등 다양한 연구용 응용에 적합합니다. 비활성화된 비인산화 CCR5에 특이적으로 결합하며 HIV 관련 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.0003 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RC-10 |
| Immunogen | This antibody was raised by immunizing mice with CCR5. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074738 |
Product Specific Information
This antibody recognizes non-activated, nonphospho-CCR5. CCR5 is a receptor for several inflammatory CC-chemokines including CCL3 (MIP-1-alpha), CCL4 (MIP-1-beta), and RANTES.
Target Information
Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. CCR5, a member of the chemokine receptor family, acts as a principal coreceptor for macrophage- and dual-tropic HIV-1 strains, enabling fusion and entry into human white blood cells. CCR5 is essential for infection by HIV-1, HIV-2, and SIV. Its ligands—RANTES, MIP-alpha, and MIP-beta—can block M-tropic HIV-1 infection. CCR5 interacts with the CD4-gp120 complex of HIV, facilitating membrane fusion and viral entry. The amino-terminal domain and extracellular loops serve as HIV binding sites. CCR5 mRNA is expressed in lymphoid organs and monocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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