Thermo Fisher Scientific HIF1A Polyclonal Antibody
HIF1A 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, IP, ChIP 등 다양한 응용에 사용 가능. 인간, 생쥐, 랫드 등 다종 반응성. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보존. 저산소 반응 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 |
| Immunohistochemistry (Frozen) (IHC-F) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:570 |
| ChIP assay (ChIP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rabbit, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human HIF1 alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.38 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792634 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (90%), Rat (90%), Dog (97%), Pig (95%), Rabbit (95%), Bovine (97%).
- Positive Control: MCF-7, HeLa (500 µM CoCl₂ for 24 hr), NIH-3T3 (500 µM CoCl₂ for 24 hr), HepG2 whole cell lysate/extract (1% O₂ treatment for 24 hr), 293T transfected HIF1A, HepG2 (200 µM CoCl₂ for 24 hr), HepG2 (500 µM CoCl₂ for 24 hr).
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
HIF1-alpha (HIF1A) is a subunit of HIF1, a transcription factor in mammalian cells under reduced oxygen tension. HIF-1 is a heterodimer composed of alpha and beta subunits, both belonging to the basic-helix-loop-helix PAS family of transcription factors. It regulates cellular response to hypoxia by activating transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, and VEGF.
HIF1-alpha modulates hypoxia-mediated apoptosis, cell proliferation, and angiogenesis. Under hypoxia, it interacts with p53 and undergoes MDM2-mediated ubiquitination and degradation. It also contributes to NOX4 induction, maintaining ROS levels after hypoxia.
The human HIF1A gene is located on chromosome 14q, and its C-terminal region binds to p300, forming complexes that regulate hypoxia-responsive genes.
Hypoxia plays a significant role in diseases such as myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease, and COPD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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