Thermo Fisher Scientific Phospho-CaMKII alpha (Thr286) Polyclonal Antibody
Rabbit polyclonal antibody recognizing phospho-CaMKII alpha (Thr286). Validated for WB, IHC(F), and ICC/IF. Suitable for mouse and rat samples. Supplied as 1 mg/mL liquid, antigen affinity purified, stored at 4°C or −20°C. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution Range |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of rat CaMKII alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4 °C short term. For long-term storage, store at −20 °C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792638 |
Product Specific Information
- Keep as concentrated solution.
- Positive control: mouse brain.
- Centrifuge briefly prior to opening the vial.
Target Information
CAMK2A (CaMKII alpha) is a serine/threonine kinase that regulates numerous cellular functions, including neuronal processes, gene expression, carbohydrate metabolism, cytoskeletal organization, and calcium homeostasis.
Autophosphorylation of threonine 286 in the presence of Ca²⁺ and calmodulin activates CAMK2A, enabling a switch from calmodulin-dependent to calmodulin-independent activity.
CaMKII is ubiquitously expressed and implicated in cell cycle regulation and transcription. It plays key roles in dendritic spine and synapse formation, neuronal plasticity, and sarcoplasmic reticulum Ca²⁺ transport in skeletal muscle. In neurons, it contributes structurally to actin cytoskeleton reorganization during plasticity by binding and bundling actin filaments in a kinase-independent manner.
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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