Thermo Fisher Scientific N-cadherin Polyclonal Antibody
Thermo Fisher Scientific의 N-cadherin Polyclonal Antibody는 인간, 마우스, 랫트에 반응하며 WB, IHC, ICC/IF 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제된 고순도 항체로, 세포 간 접착 단백질 연구에 이상적입니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human N-Cadherin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.15 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792635 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Rat (100%), Dog (100%), Chicken (100%), Bovine (100%).
- Positive Control: 293T, NT2D1, SK-N-SH, mouse brain, rat brain, A549.
- Centrifuge briefly prior to opening the vial.
Target Information
N-cadherin is a 140 kDa transmembrane protein that mediates calcium-dependent intercellular adhesion. It regulates morphogenetic movements during development and controls cell surface adhesion through homotypic interactions. Its function depends on association with the actin cytoskeleton via catenin proteins, and this association is modulated by phosphorylation of beta-catenin. N-cadherin contains five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail. It plays a crucial role in gastrulation and left-right asymmetry establishment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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