Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), Brilliant Ultra Violet 661, eBioscience

Thermo Fisher Scientific Ly-6G Monoclonal Antibody (1A8-Ly6g), Brilliant Ultra Violet 661, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Additional Conjugate Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 737
• Brilliant Violet 421, 480, 650, 786
• eFluor 450
• FITC
• Functional Grade
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 702
• Request custom conjugation available

Product Specific Information

The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G (Gr-1), a 25-kDa GPI protein expressed exclusively by neutrophils. It plays an important role in neutrophil infiltration, recruitment, and migration, and associates with B2 integrins CD11a/CD11b to modulate their function.

Unlike RB6-8C5, this clone binds specifically to Ly-6G on neutrophils and has been used for neutrophil depletion with reported antagonist activity.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse bone marrow cells, ≤ 1.0 µg/test
• Recommended Volume: 100 µL/test (cell number 10⁵–10⁸ cells/test)

Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm and is optimized for cytometers equipped with a UV (355 nm) laser. Ensure instrument compatibility before use.

Staining Panel Recommendation: When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (#SB-4401-42) or Brilliant Stain Buffer™ (#00-4409-75) to reduce non-specific interactions.

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored up to 3 days at 4°C in IC Fixation Buffer (#00-8222-49) or 1-step Fix/Lyse Solution (#00-5333-54) with minimal brightness impact.

• Excitation: 350 nm
• Emission: 660 nm
• Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

Ly6G (Lymphocyte antigen 6 complex locus G6D) is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. It is expressed by myeloid-derived cells in bone marrow and serves as a marker for neutrophils, monocytes, and granulocytes. Expression correlates with granulocyte differentiation and maturation. Ly6G may play roles in anti-tumor response and modulation of local immune responses.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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