Thermo Fisher Scientific CD235a (Glycophorin A) Monoclonal Antibody (10F7MN), Brilliant Ultra Violet 661, eBioscience

Thermo Fisher Scientific CD235a (Glycophorin A) Monoclonal Antibody (10F7MN), Brilliant Ultra Violet 661, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats

• Brilliant UV 737
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• Request custom conjugation

Product Specific Information

The monoclonal antibody 10F7MN recognizes human glycophorin A (CD235a), detecting both M and N alleles. Glycophorin A is a 151 amino acid sialoglycoprotein found on erythrocyte and erythroid progenitor cell membranes (~500,000 copies per cell). The gene resides on chromosome 4 with two allelic forms (M and N) differing by two amino acids.

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Pre-diluted and validated on normal human peripheral blood cells at 5 µL (0.5 µg) per test.

Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm and is designed for cytometers with an ultraviolet (355 nm) laser. Ensure instrument compatibility.

When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Light Sensitivity: Protect from light to avoid photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark.

Excitation: 350 nm
Emission: 660 nm
Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD235a (Glycophorin A, GPA) is a single-pass membrane sialoglycoprotein expressed in mature erythrocytes and erythroid precursor cells. It carries blood group M and N specificities and serves as a barrier to cell fusion, minimizing RBC aggregation. CD235a also acts as a receptor for Sandei virus, parvovirus, and Streptococcus adhesin Hsa.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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