Thermo Fisher Scientific TNFR1 (soluble) Polyclonal Antibody, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.5–2.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MDSVCPQGKY IHPQNNSICC TKCHKGTYLY NDCPGPGQDT DCRECESGSF TASENHLRHC LSCSKCRKEM GQVEISSCTV DRDTVCGCRK NQYRHYWSEN LFQCFNCSLC LNGTVHLSCQ EKQNTVCTCH AGFFLRENEC VSCSNCKKSL ECTKLCLPQI EN.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sTNF Receptor Type I. Anti-Human sTNF Receptor Type I-specific antibody was purified by affinity chromatography employing an immobilized Human sTNF Receptor Type I matrix.

Sandwich ELISA:
To detect Human sTNF Receptor Type I by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human sTNF Receptor Type I (500-P143Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human sTNF Receptor Type I.

Western Blot:
To detect Human sTNF Receptor Type I by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL. In conjunction with compatible secondary reagents, the detection limit for Recombinant Human sTNF Receptor Type I is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Target Information

TNFR1 (Tumor necrosis factor receptor 1) belongs to the tumor necrosis factor superfamily and is one of the major TNF-alpha receptors. TNFR1 plays an important role in cytokine-mediated signaling, positive regulation of the NF-κB pathway, angiogenesis, and gene expression regulation. The extracellular domain of TNFR1 is released into circulation as soluble TNFR1 (sTNFR1).

In humans, the TNFR1 gene is located on chromosome 12. Proteins such as BAG4/SODD, TRADD, and TRAF2 interact with TNFR1, regulating its signal transduction. Germline mutations in TNFR1 extracellular domains are associated with autosomal dominant periodic fever syndrome, where impaired receptor clearance is a proposed mechanism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.