Thermo Fisher Scientific GLI3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500- 1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

0.5-5 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

ELISA (ELISA)

1:6,000-1:30,000

-

Immunoprecipitation (IP)

1:500-1:2,000

-

Product Specifications

Species Reactivity

Avian, Dog, Chicken, Chimpanzee, Human, Non-human primate, Xenopus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This affinity purified antibody was produced from monospecific rabbit serum by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-60 of human Gli-3 protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GLI3,uniProtId:P10071-1,ncbiNodeId:9606,antigenRange:30-60,antigenLength:1580,antigenImageFileName:600-401-694_GLI3_P10071-1_Rabbit.svg,antigenImageFileNamePDP:600-401-694_GLI3_P10071-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity-purified antibody is directed against human Gli-3 protein. A BLAST analysis was used to suggest cross reactivity with Gli-3 from human, chimpanzee, squirrel monkey, Xenopus laevis, chicken, dog and quail based on 100% sequence homology with the immunogen. Reactivity is also expected against homologues from mouse (94%) and rat (88%) based on partial homology. Reactivity with Gli-3 from other sources is not known.

Target Information

This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.