Thermo Fisher Scientific GLI3 Polyclonal Antibody
GLI3 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, ELISA, IP 등 다양한 응용에 사용 가능. Affinity chromatography로 정제되었으며, 1 mg/mL 농도의 액상 제품. Avian, Human 등 여러 종에 반응하며, Shh 신호전달 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution / Concentration |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 0.5–5 µg/mL |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | 1:6,000–1:30,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Avian, Dog, Chicken, Chimpanzee, Human, Non-human primate, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to an internal region near amino acids 30–60 of human Gli-3 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid repeated freeze/thaw cycles.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This affinity-purified antibody is directed against human Gli-3 protein.
BLAST analysis suggests cross-reactivity with Gli-3 from human, chimpanzee, squirrel monkey, Xenopus laevis, chicken, dog, and quail (100% sequence homology).
Reactivity is also expected with mouse (94%) and rat (88%) based on partial homology.
Reactivity with Gli-3 from other sources is not known.
Target Information
The GLI3 gene encodes a C2H2-type zinc finger protein belonging to the Gli family, which functions as a DNA-binding transcription factor and mediator of Sonic hedgehog (Shh) signaling.
It localizes in the cytoplasm and activates the patched Drosophila homolog (PTCH) gene expression.
GLI3 plays a role in embryogenesis, and mutations in this gene are associated with several disorders such as Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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