Thermo Fisher Scientific CD209a Monoclonal Antibody (LWC06), PE, eBioscience

Applications

Tested Application

• Flow Cytometry (Flow)

Tested Dilution

• 0.5 µg/test

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Product Specifications

Additional Formats

• Unconjugated
• APC
• Biotin
• FITC
• Request custom conjugation

Product Specific Information

The LWC06 antibody was generated by immunization with the recombinant extracellular region of mouse CIRE/DC-SIGN (CD209). CIRE/DC-SIGN is a 33 kDa type II transmembrane C-type lectin protein expressed on CD8α⁻ dendritic cells and plasmacytoid predendritic cells. It is the closest homologue of human DC-SIGN, which binds HIV gp120 independently of CD4.

CIRE/DC-SIGN contains a C-terminal carbohydrate recognition domain (CRD) that binds mannose and other carbohydrates in a calcium-dependent manner. It may play a role in T cell–dendritic cell interactions through ICAM family binding. Expression varies depending on activation state and is down-regulated in GM-CSF–supplemented dendritic cell cultures. Unlike human DC-SIGN, CIRE/DC-SIGN mRNA is also detected in B cells.

The LWC06 monoclonal antibody does not cross-react with SIGNR1, SIGNR2, SIGNR3, or SIGNR4. CD209 protein is sensitive to collagenase treatment.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse CIRE/DC-SIGN-transfected CHO cells at ≤0.5 µg/test (100 µL final volume).
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene encodes a transmembrane receptor known as DC-SIGN, expressed on dendritic cells and macrophages. It functions in innate immunity by recognizing diverse pathogens, including viruses and parasites. The protein has three domains: an N-terminal transmembrane domain, a tandem-repeat neck domain, and a C-type lectin carbohydrate recognition domain.

The extracellular region acts as both a pathogen recognition and cell adhesion receptor. The neck domain mediates oligomerization, enhancing multivalent ligand binding. Variations in neck domain repeats affect ligand binding ability. It is closely related to L-SIGN (GeneID 10332), differing in ligand specificity and distribution. Multiple splice variants exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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