Thermo Fisher Scientific CD209a Monoclonal Antibody (LWC06), PE, eBioscience
마우스 CD209(CIRE/DC-SIGN)을 인식하는 PE 결합 단클론 항체로, 유세포분석(Flow Cytometry)에 적합합니다. Rat IgG2a 아이소타입이며, 0.2 mg/mL 농도의 액상 제품입니다. 4°C 암소 보관하며, 다양한 형광 표지 형식으로도 제공됩니다.
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Applications
Tested Application
- Flow Cytometry (Flow)
Tested Dilution
- 0.5 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Hamster, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | LWC06 |
| Conjugate | PE |
| Excitation/Emission Max | 565/576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_657702 |
Additional Formats
Product Specific Information
The LWC06 antibody was generated by immunization with the recombinant extracellular region of mouse CIRE/DC-SIGN (CD209). CIRE/DC-SIGN is a 33 kDa type II transmembrane C-type lectin protein expressed on CD8α⁻ dendritic cells and plasmacytoid predendritic cells. It is the closest homologue of human DC-SIGN, which binds HIV gp120 independently of CD4.
CIRE/DC-SIGN contains a C-terminal carbohydrate recognition domain (CRD) that binds mannose and other carbohydrates in a calcium-dependent manner. It may play a role in T cell–dendritic cell interactions through ICAM family binding. Expression varies depending on activation state and is down-regulated in GM-CSF–supplemented dendritic cell cultures. Unlike human DC-SIGN, CIRE/DC-SIGN mRNA is also detected in B cells.
The LWC06 monoclonal antibody does not cross-react with SIGNR1, SIGNR2, SIGNR3, or SIGNR4. CD209 protein is sensitive to collagenase treatment.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse CIRE/DC-SIGN-transfected CHO cells at ≤0.5 µg/test (100 µL final volume).
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This gene encodes a transmembrane receptor known as DC-SIGN, expressed on dendritic cells and macrophages. It functions in innate immunity by recognizing diverse pathogens, including viruses and parasites. The protein has three domains: an N-terminal transmembrane domain, a tandem-repeat neck domain, and a C-type lectin carbohydrate recognition domain.
The extracellular region acts as both a pathogen recognition and cell adhesion receptor. The neck domain mediates oligomerization, enhancing multivalent ligand binding. Variations in neck domain repeats affect ligand binding ability. It is closely related to L-SIGN (GeneID 10332), differing in ligand specificity and distribution. Multiple splice variants exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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