Thermo Fisher Scientific Mouse IgG2a kappa Isotype Control (eBM2a), Brilliant Violet 786, eBioscience
Flow cytometry용 Mouse IgG2a kappa 동형 대조 항체로 Brilliant Violet 786 형광체가 결합되어 있음. 마우스 IgG2a 항체의 비특이적 결합 확인에 사용. 광민감성으로 어둡게 보관 필요. Violet laser(405 nm) 기반 분석에 적합.
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Thermo Fisher Scientific Mouse IgG2a kappa Isotype Control (eBM2a), Brilliant Violet 786
Applications
- Flow Cytometry (Flow): Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBM2a |
| Conjugate | Brilliant Violet™ 786 |
| Excitation/Emission Max | 406 / 788 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925741 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 563, 615, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711
- eFluor 450, 506, 615, 660
- FITC
- Functional Grade
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation available
Product Specific Information
This monoclonal mouse IgG2a kappa antibody is used as an isotype control for mouse IgG2a antibodies.
Reported for use in flow cytometric analysis. Tested with normal human peripheral blood and mouse splenocytes. Use at the same concentration as the experimental antibody.
Brilliant Violet™ 786 (BV786) is a tandem dye emitting at 786 nm, suitable for cytometers equipped with a violet (405 nm) laser. Ensure instrument compatibility.
Buffer recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.
Light sensitivity:
BV786 is sensitive to photo-induced oxidation. Protect vial and samples from light.
Fixation compatibility:
Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark. BV786 is not compatible with methanol-based fixation.
Excitation/Emission:
407 nm / 786 nm (Violet Laser)
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
Mouse IgG2a is a subtype of immunoglobulin G produced by B cells in response to antigens. It plays a key role in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity.
Widely used in research applications such as ELISA, Western blotting, and immunohistochemistry, as well as in immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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