Thermo Fisher Scientific Mouse IgG2b kappa Isotype Control (eBMG2b), Brilliant Violet 786, eBioscience
Flow cytometry용 Mouse IgG2b kappa 동형 대조항체로, Brilliant Violet 786 형광체 결합. 비특이적 결합 평가 및 대조 실험에 적합. Violet 레이저(405 nm) 사용 장비에 최적화. 광민감성이 있어 암조건 보관 필요.
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Applications
- Flow Cytometry (Flow): Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBMG2b |
| Conjugate | Brilliant Violet™ 786 |
| Excitation / Emission Max | 406 / 788 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925742 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 563, 615, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711
- eFluor 450, 506, 570, 615, 660
- FITC
- Functional Grade
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation available
Product Specific Information
Description:
Monoclonal mouse IgG2b kappa is useful as an isotype control immunoglobulin.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood and mouse splenocytes.
Use at the same concentration as the experimental antibody.
Fluorochrome Information:
Brilliant Violet™ 786 (BV786) is a tandem dye emitting at 786 nm, optimized for cytometers equipped with a violet (405 nm) laser. Ensure instrument compatibility before use.
Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions.
Light Sensitivity:
Sensitive to photo-induced oxidation. Protect from light during storage and use.
Fixation Compatibility:
Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
Not compatible with methanol-based fixation.
Excitation / Emission / Laser:
407 nm / 786 nm / Violet Laser
Trademark Notice:
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.
Target Information
Mouse IgG2b is a subtype of immunoglobulin G antibody produced by B cells in response to antigens.
It is the third most abundant IgG subtype in serum and plays a key role in adaptive immunity, including opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity.
It is used widely in research applications such as ELISA, Western blotting, and immunohistochemistry, as well as in therapeutic research including immunotherapy and vaccine development.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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