Thermo Fisher Scientific Rat IgG1 kappa Isotype Control (eBRG1), Brilliant Violet 786, eBioscience
Brilliant Violet 786으로 표지된 Rat IgG1 kappa 동형 대조 항체로, 유세포 분석용 음성 대조로 사용됩니다. Violet 레이저(405 nm) 기반 형광 검출에 적합하며, 비특이적 결합 최소화를 위해 Brilliant Stain Buffer 사용을 권장합니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific Rat IgG1 kappa Isotype Control (eBRG1), Brilliant Violet 786
Applications and Tested Dilution
- Flow Cytometry (Flow): Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Rat / IgG1, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBRG1 |
| Conjugate | Brilliant Violet™ 786 |
| Excitation/Emission Max | 406 / 788 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925740 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 563, 615, 661, 737, 805
- Brilliant Violet 421, 480, 605, 650, 711
- eFluor 450, 506, 570, 615, 660
- FITC
- Functional Grade
- Pacific Orange
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation
Product Specific Information
The monoclonal rat IgG1 kappa is useful as an isotype control immunoglobulin.
- Applications Reported: Suitable for flow cytometric analysis.
- Applications Tested: Tested on normal human peripheral blood and mouse splenocytes. Use at the same concentration as the experimental antibody.
- Fluorochrome Information: Brilliant Violet™ 786 (BV786) emits at 786 nm and is optimized for cytometers equipped with a violet (405 nm) laser.
- Buffer Recommendation: When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions.
- Light Sensitivity: Protect vial and stained samples from light due to photo-induced oxidation.
- Fixation Compatibility: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) up to 3 days at 4°C in the dark. BV786 is not compatible with methanol-based fixation.
- Excitation/Emission/Laser: 407 nm / 786 nm / Violet Laser.
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
Target Information
Rat IgG1 is a subclass of IgG antibodies produced by rats in response to antigens. Commonly used as a negative control in experiments, as it does not bind to many mammalian antigens. Utilized in immunohistochemistry, flow cytometry, and western blotting to detect specific antigens. Rat IgG1 also plays a role in gene targeting studies for disease model generation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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