Thermo Fisher Scientific H3.3 K36M oncohistone mutant Recombinant Polyclonal Antibody (10HCLC), ChIP-Verified

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250

-

ChIP assay (ChIP)

1-5 µg

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Recombinant Superclonal

Type

Antibody

Clone

10HCLC

Immunogen

Peptide corresponding to human H3.3 K36M [aa32-aa40]

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2848519

Product Specific Information

This antibody is predicted to react with Mouse, Rat, Bovine.

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

Target Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.