Thermo Fisher Scientific H3K4me3 Recombinant Superclonal Antibody (9HCLC), ChIP-Verified
ChIP 검증된 H3K4me3 표적 리콤비넌트 Superclonal 항체로 높은 특이성과 감도를 제공. 인간, 마우스, 랫트, 효모 반응성. WB, ICC/IF, ChIP, ChIP-Seq, Peptide Array 등 다양한 응용 가능. 재조합 기술로 로트 간 일관성 확보.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:250–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ChIP assay (ChIP) | 1–5 µg |
| ChIP-sequencing (ChIP-Seq) | 3 µg |
| Peptide Array (Array) | 0.25 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Yeast |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Superclonal |
| Type | Antibody |
| Clone | 9HCLC |
| Immunogen | Peptide corresponding to human Tri-Methyl-Histone H3 (Lys 4) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2848518 |
Product Specific Information
This antibody is predicted to react with Rat.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They consist of multiple different recombinant monoclonal antibodies, combining the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies, and offering the consistency of recombinant production.
These antibodies recognize multiple epitope sites on the target, providing higher detection sensitivity for low-abundance targets. Unlike traditional polyclonal antibodies, recombinant Superclonal™ antibodies have a defined mixture of light and heavy chains, ensuring reproducibility across lots and eliminating biological variability.
Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and performance remain unchanged.
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of eukaryotic cells. Together with other core histones, it forms the nucleosome structure. Histones play key roles in transcription regulation, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 can undergo post-translational modifications such as methylation, acetylation, and phosphorylation, which influence chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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