Thermo Fisher Scientific H3K4me2 Recombinant Superclonal Antibody, ChIP-Verified
ChIP 검증된 H3K4me2 Superclonal™ 항체로 높은 특이성과 감도를 제공. 재조합 토끼 IgG 포맷으로 로트 간 일관성 확보. WB, ICC, IHC, ChIP 등 다양한 응용에 적합. 저항체 변동성이 적고 신뢰성 높은 데이터 생성에 최적화.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–1 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | – |
| ChIP assay (ChIP) | 1–5 µg | View 1 publication |
| Peptide Array (Array) | 0.25 µg/mL | – |
| CUT&RUN (C&R) | 1:100 | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Yeast |
| Published Species | C. elegans, Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Superclonal |
| Type | Antibody |
| Immunogen | Methylated peptide (Lys4) corresponding to human Histone H3 (aa 2–10) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2848516 |
Product Specific Information
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They consist of multiple different recombinant monoclonal antibodies, combining the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies, and the consistency of recombinant production.
Functionally similar to polyclonal antibodies, they recognize multiple epitopes on the target, providing higher detection sensitivity for low-abundance targets. Each lot is produced from a known mixture of light and heavy chains, minimizing biological variability typical of polyclonal antibody production.
Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and performance remain unchanged.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. It forms nucleosomes with other core histones and plays a central role in transcription regulation, DNA repair, replication, and chromosomal stability. The N-terminal tail of histone H3 undergoes post-translational modifications such as methylation, acetylation, and phosphorylation, influencing chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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