Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), eBioscience
Mouse CD11b 단백질을 인식하는 Rat IgG2b 단클론 항체로, Flow cytometry, IHC, IP 등 다양한 분석에 활용 가능. Mac-1 복합체 검출에 적합하며 human CD11b에도 교차 반응. 고순도(>90%)로 제조되어 연구용으로 안정적 사용 가능.
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Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 3 |
| Immunohistochemistry (IHC) | - | 63 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | 1 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 5 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 10 |
| Immunocytochemistry (ICC/IF) | - | 35 |
| Flow Cytometry (Flow) | 0.25 µg/test | 541 |
| ELISA | - | 1 |
| Immunoprecipitation (IP) | Assay-Dependent | 2 |
| Neutralization (Neu) | Assay-Dependent | 7 |
| Functional Assay | Assay-Dependent | 3 |
| Inhibition Assays (IA) | - | 1 |
| In vitro Assay (IV) | - | 2 |
| Miscellaneous PubMed | - | 7 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Bat, Fish, Human, Mouse, Rabbit |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M1/70 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467107 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Conjugated Formats
- Alexa Fluor 488, 532, 660, 700
- APC, APC-eFluor 780, Biotin
- Brilliant UV 395–805 series
- Brilliant Violet 421–786 series
- eFluor 450, 506, 660
- FITC, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436–780 series
- NovaFluor Blue/Yellow series
- Functional Grade format available
- Custom conjugation available on request
Product Specific Information
The M1/70 monoclonal antibody reacts with mouse CD11b, a 165–170 kDa integrin alphaM that forms a non-covalent complex with CD18 (Mac-1, αMβ2 integrin). It binds CD54 (ICAM-1), C3bi, and fibrinogen. CD11b is expressed by macrophages, NK cells, granulocytes, activated lymphocytes, and B-1 cells in the peritoneal cavity. M1/70 also cross-reacts with human CD11b and can detect this antigen on human monocytes, granulocytes, and NK cells.
Applications Reported:
- Flow cytometry
- Immunoprecipitation
- Immunohistochemical staining of frozen tissue
- In vitro blocking of CD11b function (use Functional Grade purified M1/70 in functional assays)
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes at ≤0.25 µg/test (100 µL final volume). Optimal antibody titration recommended.
Target Information
CD11b (integrin αM, ITGAM) is a 165 kDa adhesion molecule forming a heterodimer with CD18 (integrin β2). The CD11b/CD18 complex (Mac-1, CR3) acts as a receptor for ICAM-1, ICAM-2, CD14, fibrinogen, and iC3b, mediating cell-cell and cell-matrix interactions. It is expressed on monocytes/macrophages, granulocytes, NK cells, dendritic cells, and microglia. CD11b expression increases during monocyte maturation and varies across macrophage populations. Associated with systemic lupus erythematosus and ITGAM-related susceptibility disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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