Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), eBioscience

Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugated Formats

• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780, Biotin
• Brilliant UV 395–805 series
• Brilliant Violet 421–786 series
• eFluor 450, 506, 660
• FITC, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436–780 series
• NovaFluor Blue/Yellow series
• Functional Grade format available
• Custom conjugation available on request

Product Specific Information

The M1/70 monoclonal antibody reacts with mouse CD11b, a 165–170 kDa integrin alphaM that forms a non-covalent complex with CD18 (Mac-1, αMβ2 integrin). It binds CD54 (ICAM-1), C3bi, and fibrinogen. CD11b is expressed by macrophages, NK cells, granulocytes, activated lymphocytes, and B-1 cells in the peritoneal cavity. M1/70 also cross-reacts with human CD11b and can detect this antigen on human monocytes, granulocytes, and NK cells.

Applications Reported:

• Flow cytometry
• Immunoprecipitation
• Immunohistochemical staining of frozen tissue
• In vitro blocking of CD11b function (use Functional Grade purified M1/70 in functional assays)

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes at ≤0.25 µg/test (100 µL final volume). Optimal antibody titration recommended.

Target Information

CD11b (integrin αM, ITGAM) is a 165 kDa adhesion molecule forming a heterodimer with CD18 (integrin β2). The CD11b/CD18 complex (Mac-1, CR3) acts as a receptor for ICAM-1, ICAM-2, CD14, fibrinogen, and iC3b, mediating cell-cell and cell-matrix interactions. It is expressed on monocytes/macrophages, granulocytes, NK cells, dendritic cells, and microglia. CD11b expression increases during monocyte maturation and varies across macrophage populations. Associated with systemic lupus erythematosus and ITGAM-related susceptibility disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.