Thermo Fisher Scientific HSF2 Monoclonal Antibody (OTI2A7), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Prokaryotic and eukaryotic cells respond to thermal and chemical stress by inducing a group of heat shock genes. In eukaryotes, transcriptional regulation is primarily controlled by heat shock transcription factors (HSF1 and HSF2).
HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein levels. HSF1 normally exists as a monomer with repressed transcriptional activity due to constitutive phosphorylation. Upon activation, HSF1 forms trimers, binds DNA, and translocates to the nucleus.
HSF2 is associated with differentiation and development, and like HSF1, binds DNA as a trimer. Both factors are induced by proteasome inhibitors within the ubiquitin pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.