Thermo Fisher Scientific Macrophages (Mature) Monoclonal Antibody (25F9), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

Immunohistochemistry (Frozen) (IHC (F))

1:50

-

Flow Cytometry (Flow)

1:50

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

25F9

Immunogen

The monoclonal antibody 25F9 recognizes a protein of 86 kD on the cell surface and within the cytoplasm of mature macrophages.

Conjugate

Biotin Biotin Biotin

View additional formats

• Unconjugated
• FITC
• Request custom conjugation

Form

Liquid

Concentration

100 µg/mL

Purification

Protein G

Storage buffer

PBS with 0.1% BSA

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_3090506

Product Specific Information

The antibody is associated with fully differentiated tissue macrophages both in normal and in diseased tissues, independently of the presence or absence of inflammation. The antigen is absent on freshly isolated monocytes and other blood cells. After 6 to 7 days culture human monocytes become positive. Some melanoma and carcinoma cell lines are also positive. Furthermore the monoclonal antibody 25F9 cross reacts with a subpopulation of macrophages of rhesus monkey, pig alveolar macrophages and Kupffer cells.

Target Information

Macrophages are a type of white blood cell that plays a crucial role in the immune system. They are responsible for detecting, engulfing, and destroying foreign substances, such as bacteria and viruses, as well as dead cells and debris. Mature macrophages are found in various tissues throughout the body and are highly specialized to perform specific functions depending on their location. For example, alveolar macrophages are found in the lungs and are responsible for removing foreign particles that are inhaled, while Kupffer cells are found in the liver and are responsible for removing old or damaged red blood cells. Understanding the function and behavior of macrophages is crucial for developing new treatments for diseases such as cancer, autoimmune disorders, and infections.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.