Thermo Fisher Scientific Macrophages (Mature) Monoclonal Antibody (25F9)
성숙 대식세포 표면 단백질(86 kD)을 인식하는 마우스 단클론 항체. Western blot, IHC(P/F), Flow cytometry에 사용 가능. 인간 대식세포에 특이적으로 반응하며, 다양한 조직 연구에 적합. 비결합형 항체로 4°C에서 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:50 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50 |
| Flow Cytometry (Flow) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 25F9 |
| Immunogen | The monoclonal antibody 25F9 recognizes a protein of 86 kD on the cell surface and within the cytoplasm of mature macrophages. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 100 µg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 0.1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_3090344 |
Additional Formats
Product Specific Information
The antibody is associated with fully differentiated tissue macrophages both in normal and diseased tissues, independently of the presence or absence of inflammation. The antigen is absent on freshly isolated monocytes and other blood cells. After 6 to 7 days of culture, human monocytes become positive. Some melanoma and carcinoma cell lines are also positive. Furthermore, the monoclonal antibody 25F9 cross-reacts with a subpopulation of macrophages of rhesus monkey, pig alveolar macrophages, and Kupffer cells.
Target Information
Macrophages are a type of white blood cell that plays a crucial role in the immune system. They detect, engulf, and destroy foreign substances such as bacteria, viruses, and dead cells. Mature macrophages are found in various tissues throughout the body and are specialized for tissue-specific functions.
- Alveolar macrophages: Found in the lungs, remove inhaled particles.
- Kupffer cells: Found in the liver, remove old or damaged red blood cells.
Understanding macrophage function is vital for research into cancer, autoimmune disorders, and infections.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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