Thermo Fisher Scientific RAD23B Monoclonal Antibody (OTI7G10), TrueMAB
RAD23B 단백질을 인식하는 Mouse monoclonal antibody로 Western blot과 IHC(P)에서 검증됨. 인간 단백질에 반응하며, 동결건조 형태로 공급됨. PBS buffer에 8% trehalose 포함, 보존제 무첨가. 연구용으로 DNA 손상 복구 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI7G10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–253 of human RAD23B produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The RAD23B protein is one of two human homologs of Saccharomyces cerevisiae Rad23, involved in nucleotide excision repair (NER).
It complements the NER defect in xeroderma pigmentosum group C (XP-C) cell extracts and interacts with 3-methyladenine-DNA glycosylase (MPG), suggesting a role in DNA damage recognition in base excision repair.
The protein contains an N-terminal ubiquitin-like domain that interacts with the 26S proteasome, indicating involvement in the ubiquitin-mediated proteolytic pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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