Thermo Fisher Scientific RAD23B Monoclonal Antibody (OTI9F8), TrueMAB
RAD23B 단백질을 표적하는 Mouse IgG1 단클론 항체로, Western blot 및 IHC(P) 실험에 최적화됨. 인간 RAD23B 재조합 단백질(aa 1-253)을 면역원으로 사용. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도. DNA 손상 복구 연구에 적합.
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Applications
- Western Blot (WB): 1:2,000
- Immunohistochemistry (Paraffin) (IHC (P)): 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI9F8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–253 of human RAD23B produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
The RAD23B gene encodes one of two human homologs of Saccharomyces cerevisiae Rad23, involved in nucleotide excision repair (NER).
RAD23B complements the NER defect in xeroderma pigmentosum group C (XP-C) cell extracts and interacts with 3-methyladenine-DNA glycosylase (MPG), suggesting a role in DNA damage recognition during base excision repair.
It contains an N-terminal ubiquitin-like domain that interacts with the 26S proteasome, implicating RAD23B in the ubiquitin-mediated proteolytic pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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