Thermo Fisher Scientific Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4), Alexa Fluor 514
Alexa Fluor 514으로 표지된 Alpha-Smooth Muscle Actin 단일클론 항체(1A4). 사람, 마우스, 랫트 시료에 반응하며 IHC(P)에서 1 µg/mL로 최적화됨. 평활근세포 및 근섬유성 세포 마커로 사용되며, 형광 분석에 적합. 4°C 암소 보관.
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Thermo Fisher Scientific Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4), Alexa Fluor 514
Applications and Tested Dilution
- Immunohistochemistry (Paraffin) (IHC (P)): 1 µg/mL
(Tested using high pH antigen retrieval)
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1A4 |
| Conjugate | Alexa Fluor™ 514 |
| Excitation/Emission Max | 518 / 543 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 0.5% BSA, 10% proprietary stabilizer |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
Additional Formats
- Unconjugated
- Alexa Fluor 420
- Alexa Fluor 700
- Alexa Fluor Plus 594
- Alexa Fluor Plus 750
- Request custom conjugation available
Product Specific Information
The monoclonal antibody 1A4 recognizes human, mouse, and rat alpha-smooth muscle actin, a 42 kDa cytoskeletal protein. It is specific for the alpha form expressed in smooth muscle cells of blood vessels, myofibroblasts, and myoepithelial cells.
Useful for identifying leiomyomas, leiomyosarcomas, and pleomorphic adenomas, and as a prognostic marker for basal cell carcinoma.
Applications Reported: Immunohistochemical staining (FFPE tissues) and immunocytochemistry.
Applications Tested: IHC on formalin-fixed paraffin-embedded tissue using high pH antigen retrieval (1 µg/mL recommended).
Careful titration advised for optimal performance.
Using Conjugate Solutions:
Before use, centrifuge briefly and use only the supernatant to remove aggregates and reduce nonspecific background staining.
Target Information
Smooth Muscle Actin is a member of the actin protein family, essential for cell motility, structure, and integrity.
- Isoforms: Alpha, beta, gamma actins
- Alpha actin: Major component of contractile apparatus
- Beta/gamma actins: Regulate cell motility
Smooth muscle actin is found in:
- Vessel walls, gut wall, myometrium
- Myoepithelial cells (breast, salivary glands)
- Stress fibers of fibroblastic cells during wound healing and fibrocontractive diseases
Defects in the smooth muscle actin gene are associated with familial thoracic aortic aneurysm type 6.
Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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