Thermo Fisher Scientific CD144 (VE-cadherin) Monoclonal Antibody (eBioBV13 (BV13)), Functional Grade, eBioscience
마우스 VE-Cadherin(CD144)을 인식하는 단클론 항체로, 혈관내피세포 간 접착 연구에 적합합니다. Flow cytometry, WB, IHC 등 다양한 응용에 사용 가능하며, Functional Grade로 제조되어 세포 실험에 적합합니다. 고순도(>90%) 및 저엔도톡신(<0.001 ng/µg)으로 품질 보장.
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4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD144 (VE-cadherin) Monoclonal Antibody (eBioBV13 (BV13)), Functional Grade, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | 4 publications |
| Immunohistochemistry (IHC) | - | 5 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | - | 6 publications |
| Flow Cytometry (Flow) | 1 µg/test | 11 publications |
| ELISA | - | 1 publication |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| ChIP assay (ChIP) | - | 1 publication |
| Functional Assay | Assay-Dependent | 1 publication |
| Peptide Array | - | 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioBV13 (BV13) |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2865876 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC
- Biotin
- eFluor 450
- eFluor 660
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 600
- Request custom conjugation available
Product Specific Information
Description:
The BV13 monoclonal antibody reacts with mouse VE-Cadherin (CD144), a 120 kDa member of the type II Cadherin family. VE-Cadherin mediates homophilic adhesion between endothelial cells and localizes at adherens junctions. It is constitutively expressed throughout the vasculature and is essential for endothelial cell functions such as migration, survival, growth inhibition, and tubular structure formation. VE-Cadherin⁺CD45⁻ cells from the yolk sac or AGM region may give rise to hematopoietic cells.
Applications Reported:
This antibody has been reported for use in flow cytometry, immunoprecipitation, Western blot, and frozen tissue immunohistology. Intravenous injection of BV13 induces a concentration- and time-dependent increase in vascular permeability in heart and lung tissue.
Applications Tested:
Tested by flow cytometric analysis of bEnd.3 cells. Recommended ≤1 µg/test (in 100 µL final volume). Optimal titration is advised for each assay.
Storage and Handling:
Use in a sterile environment. 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE)
Endotoxin Level: <0.001 ng/µg antibody (LAL assay)
Aggregation: <10% (HPLC)
Target Information
VE-cadherin is a calcium-dependent cell-cell adhesion glycoprotein, part of the cadherin superfamily located on chromosome 16. It contains five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail. VE-cadherin mediates homophilic adhesion and plays a critical role in endothelial cell cohesion and junction organization. An alternative splice variant has been reported, though its full sequence is not yet determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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