Thermo Fisher Scientific CD140b (PDGFRB) Monoclonal Antibody (APB5), Functional Grade, eBioscience

Thermo Fisher Scientific CD140b (PDGFRB) Monoclonal Antibody (APB5), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• Biotin
• PE
• PE-Cyanine7
• PE-eFluor 610
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Custom conjugation available upon request

Product Specific Information

The APB5 monoclonal antibody reacts with mouse CD140b (PDGFRβ), the beta chain of the platelet-derived growth factor receptor. PDGFRβ is a receptor tyrosine kinase forming dimers (α/α, β/β, or α/β) upon ligand binding and phosphorylates substrates. CD140b is expressed in embryonic tissues and mesenchymal-derived adult cells. PDGFRβ plays a key role in fibrous atherosclerotic lesion formation.

Applications Reported:

• Flow cytometric analysis
• Blocking of ligand binding

Applications Tested:

• Tested by flow cytometric analysis of NIH/3T3 cells
• Recommended ≤1 µg/test (100 µL final volume)
• Cell number: 10⁵–10⁸ cells/test
• Optimize antibody titration for best assay performance

Storage and Handling:

• Use in sterile environment
• 0.2 µm post-manufacturing filtered

Purity: >90% (SDS-PAGE)
Endotoxin Level: <0.001 ng/µg antibody (LAL assay)
Aggregation: <10% (HPLC)

Target Information

PDGFRB is a receptor tyrosine kinase implicated in atherogenesis and oncogenic transformation by SIS. It acts as a mitogen for mesenchymal-origin cells. The receptor’s dimer composition (αα, ββ, or αβ) depends on the bound growth factor. On chromosome 5, PDGFRB is flanked by GM-CSF and M-CSFR genes, associated with 5-q syndrome. A translocation between chromosomes 5 and 12 fusing PDGFRB to ETV6 leads to chronic myeloproliferative disorder with eosinophilia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.