Thermo Fisher Scientific Proteinase K Recombinant Protein

Applications

• Suitable for protein digestion and nucleic acid preparation assays
• Activity tested and assay-dependent

Product Specifications

Product Specific Information

• Animal Origin Free
• No DNase contamination detected by digestion of lambda DNA at 37°C for 6 hours
• No RNase contamination detected by digestion of lambda DNA at 25°C for 16 hours
• No host genomic DNA/RNA residual detected by PCR
• Storage: Lyophilized protein stable at 4°C for up to 1 year; for long-term, store at -20°C
• Recommended reconstitution buffer: 20 mM Tris-HCl, 30 mM NaCl, 50% Glycerol, 5 mM CaCl₂, pH 7.2–7.4
• After reconstitution: stable at -20°C for 1 year, at 2–8°C for up to 6 months

Target Information

Proteinase K (E.C.3.4.21.64) is an extracellular serine endoproteinase produced by Tritirachium album Limber.
It is active over a wide range of temperatures and buffers, with optimal activity between 20°C and 60°C and pH 7.5–12.0.
Activity is enhanced by up to 2% SDS or 4 M urea. Calcium contributes to thermostability but is not required for catalysis, allowing activity in buffers containing chelating agents such as EDTA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.