Thermo Fisher Scientific Proteinase K Recombinant Protein

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Fungi

Expression System

P. pastoris

Amino acid sequence

Full length Proteinase K protein

Class

Recombinant

Type

Protein

Purity

>95% by SDS-Page

Activity

Measured by its digestion activity. The activity is >30 U/mg. One unit will digest Casein at 37°C (pH 7.5) per minute to produce equal absorbance as 1.0 µmol of L-tyrosine.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

Product Specific Information

Animal Origin Free

No DNase contamination detected by digestion of lambda DNA at 37°C for 6 hours. No RNases contamination detected by digestion of lambda DNA at 25°C for 16 hours. Host genomic DNA/RNA is no residual detected by PCR.

Storage: Store the lyophilized protein at 4°C for up to 1 year and less than -20°C for long term. Lyophilized powder is recommended to dissolve in in 20mM Tris-Hcl, 30mM NaCl, 50% Glycerol, 5mM CaCl2, pH 7.2-7.4. After reconstitution, the protein solution is stable at -20°C for 1 year, at 2-8°C for up to 6 months.

Target Information

Proteinase K (E.C.3.4.21.64) is an extracellular serine endoproteinase produced by Tritirachinm album Limber. Proteinase K is active in a wide range of temperatures and buffers with optimal activity between 20 and 6°C and a pH between 7.5 and 12.0. Activity is stimulated when up to 2% SDS or up to 4 M urea are included in the reaction. Calcium is important for thermostability of Proteinase K but it is not required for catalysis, therefore Proteinase K is also active in buffers containing chelating agents such as EDTA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.