Thermo Fisher Scientific Human ACE2 (aa18-740) Fc Chimera Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

HEK293 cells

Amino acid sequence

Human ACE2, amino acids Gln18-Ser740 with a C-terminal Fc-tag

Tag

Fc-tag

Molecular weight

110 kDa

Class

Recombinant

Type

Protein

Purity

>95% by SDS-PAGE

Endotoxin concentration

<0.1 EU/µg

Activity

Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 Spike RBD at 1 µg/mL (100 µL/well) can bind Human ACE2 with a linear range of 0.01-2.4 ng/mL.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

Product Specific Information

Animal Origin Free

Storage and Reconstitution: Store the lyophilized protein at -20°C to -80°C for long term. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Target Information

Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.