Thermo Fisher Scientific Human ACE2 (aa18-740), His-tag Recombinant Protein
Human ACE2 단백질(aa18-740, His-tag)로 SARS-CoV-2 스파이크 단백질 결합 연구에 적합. HEK293 세포에서 발현된 고순도 재조합 단백질로, >95% 순도. ELISA, WB, 기능 분석 등 다양한 실험에 활용 가능. 동물유래 성분 없음.
- 카탈로그번호
- RP87703
- 카테고리
- Protein
- 판매단위
- pk
카탈로그
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Applications
Western Blot (WB)
- Tested Dilution: Assay-dependent
ELISA
- Tested Dilution: Assay-dependent
Functional Assay
- Tested Dilution: Assay-dependent
Control (Ctrl)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | HEK293 cells |
| Amino Acid Sequence | Human ACE2, amino acids Gln18–Ser740 with a C-terminal His-tag |
| Tag | His-tag |
| Molecular Weight | 110–120 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% by SDS-PAGE |
| Endotoxin Concentration | <0.1 EU/µg |
| Activity | Immobilized recombinant Human ACE2 at 2 µg/mL (100 µL/well) can bind recombinant SARS-CoV-2 S1 Spike Protein with a linear range of 0.49–9.31 ng/mL |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
Product Specific Information
- Animal Origin Free
- Storage and Reconstitution:
Store the lyophilized protein at -20°C to -80°C for long term.
Reconstitute to a concentration of 0.1–0.5 mg/mL in sterile distilled water.
After reconstitution, the protein solution is stable at -20°C for 3 months, and at 2–8°C for up to 1 week.
Target Information
Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology.
In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis.
Recently, ACE2 has been shown to be a functional receptor of the SARS coronavirus.
The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII.
While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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