Thermo Fisher Scientific 4E-BP2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human 4E-BP2. Recombinant protein control fragment (Product #RP-102764). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:4E-BP2,uniProtId:Q13542-1,ncbiNodeId:9606,antigenRange:81-115,antigenLength:120,antigenImageFileName:PA5-57941_4E-BP2_Q13542-1_Rabbit.svg,antigenImageFileNamePDP:PA5-57941_4E-BP2_Q13542-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2637493

Product Specific Information

Immunogen sequence: VTSPGTLIED SKVEVNNLNN LNNHDRKHAV GDDAQ

Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 91%.

Target Information

PHAS-II (eIF4E-binding protein II) is involved in translation initiation. PHAS-II is highest in liver and kidney. PHAS proteins bind to eIF-4E, the mRNA cap-binding protein, and inhibit translation of capped mRNA in vitro and in cells. Phosphorylation in the appropriate site(s) promotes dissociation of PHAS/eIF-4E complexes. This allows eIF-4E to bind to eIF-4G (p220), thereby increasing the amount of the eIF-4F complex and the rate of translation initiation. Pharmacological and genetic evidence indicates that the mTOR/p70S6K pathway is involved in the control of PHAS-I and -II. Thus, PHAS proteins may be mediators of the effects of this pathway on protein synthesis and cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.