Thermo Fisher Scientific 4E-BP2 Polyclonal Antibody
Human 4E-BP2 단백질을 인식하는 Rabbit Polyclonal Antibody로, IHC(P)에서 1:200~1:500 희석 비율로 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS와 40% glycerol buffer에 보관. 연구용으로만 사용.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:200–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human 4E-BP2. Recombinant protein control fragment (Product #RP-102764) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2637493 |
Product Specific Information
- Immunogen sequence: VTSPGTLIED SKVEVNNLNN LNNHDRKHAV GDDAQ
- Highest antigen sequence identity to orthologs:
- Mouse: 94%
- Rat: 91%
Target Information
PHAS-II (eIF4E-binding protein II) is involved in translation initiation. PHAS-II is highly expressed in liver and kidney. PHAS proteins bind to eIF-4E, the mRNA cap-binding protein, and inhibit translation of capped mRNA in vitro and in cells. Phosphorylation at appropriate sites promotes dissociation of PHAS/eIF-4E complexes, allowing eIF-4E to bind eIF-4G (p220) and increase the rate of translation initiation. The mTOR/p70S6K pathway regulates PHAS-I and -II, suggesting PHAS proteins mediate effects on protein synthesis and cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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