Thermo Fisher Scientific CD3/CD19 Antibody Cocktail, FITC, PE

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Neat

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2a

Class

Cocktail

Type

Antibody

Clone

6D5/KT3

Immunogen

Human K562 cell line transfected with murine CD19,

Conjugate

FITC , PE FITC , PE FITC , PE

Form

Lyophilized

Concentration

Conc. Not Determined

Purification

purified

Storage buffer

PBS with 1% BSA, 5% sucrose

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538042

Product Specific Information

MA5-16537 contains a monoclonal mixture of FITC-conjugated CD19 (clone #6D5, Rat IgG2a) and RPE-conjugated CD3 (clone #KT3, Rat IgG2a). This antibody is a dual-color reagent recognizing mouse CD19 and CD3. Clone 6D5 recognizes mouse CD19, a 95 kDa cell surface glycoprotein expressed by B lymphocytes. Clone KT3 recognizes mouse CD3, a cell surface glycoprotein expressed by mature T cells.

Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4ºC in the dark.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Target Information

CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lamda light chains. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation. It is expressed in all B cell precursor leukemias.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.