Thermo Fisher Scientific Ly-6B.2 Monoclonal Antibody (7/4), FITC
Flow Cytometry용 FITC 결합 Rat anti-Mouse Ly-6B.2 단클론 항체. Neutrophil, monocyte, macrophage 표지에 적합. 498/517 nm 형광 특성으로 FACS 분석에 최적화. PBS + 1% BSA, 0.09% sodium azide 완충액에 보관. 4°C 암소 보관, 동결 금지.
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Applications
Flow Cytometry (Flow)
- Tested Dilution: Neat–1:10
- Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7/4 |
| Immunogen | Cultured bone marrow cells |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538045 |
Available Formats
- Unconjugated (Cat. MA5-16539)
- Biotin (Cat. MA5-28468)
- PE (Cat. MA5-16542)
- Custom conjugation available upon request
Product Specific Information
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
Rat anti-Mouse Ly-6B.2 monoclonal antibody, clone 7/4 recognizes the Ly-6B.2 antigen.
Target Information
Ly-6B.2 recognizes the Ly-6B.2 antigen, a 25–30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes, and some activated macrophages.
High expression levels are found in bone marrow, spleen, lung, and lymph nodes.
N-glycanase treatment of thioglycollate-elicited peritoneal neutrophil lysates reduces the apparent molecular weight to approximately 15 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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