Thermo Fisher Scientific Ly-6B.2 Monoclonal Antibody (7/4)
Mouse Ly-6B.2 항원을 인식하는 Rat 모노클로날 항체(클론 7/4)로, 저엔도톡신·무보존제 제형. WB, IHC, ICC, Flow Cytometry 등에 적합. FACS 분석 시 1×10⁶ 세포당 10 µL 사용 권장. 연구용으로만 사용 가능.
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Thermo Fisher Scientific Ly-6B.2 Monoclonal Antibody (7/4)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7/4 |
| Immunogen | Cultured bone marrow cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538044 |
Additional Formats
Product Specific Information
- Low-endotoxin, preservative-free formulation (Endotoxin level <0.01 EU/µg).
- For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
- Rat anti-Mouse Ly-6B.2 monoclonal antibody, clone 7/4 recognizes the Ly-6B.2 antigen.
Target Information
Ly-6B.2 is a 25–30 kDa, GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes, and some activated macrophages. High expression levels are observed in bone marrow, spleen, lung, and lymph nodes.
N-glycanase treatment of thioglycollate-elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to approximately 15 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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