Thermo Fisher Scientific Gata-3 Monoclonal Antibody (TWAJ), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific Gata-3 Monoclonal Antibody (TWAJ), PerCP-eFluor 710, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: 5 µL (0.06 µg)/test
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Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• Brilliant UV 395, 563, 661
• Brilliant Violet 421, 480, 605, 711, 786
• eFluor 450, eFluor 660
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• Custom conjugation available

Product Specific Information

The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a member of the Gata family of transcription factors.
Gata-3 is a T cell-specific transcription factor essential for thymic development and Th2 differentiation. Expression during embryonic development occurs in the central nervous system, skin, mammary glands, and kidney. Homozygous knockout of Gata-3 is embryonic lethal.

Gata-3 is crucial for T cell commitment and survival, mainly expressed on CD4 single positive thymocytes. During Th2 differentiation, Gata-3 binds to the IL-4 promoter and represses T-bet expression, inhibiting Th1 differentiation.

Alternative splice variants (50 and 45 kDa) have been reported, notably in MCF7 cells, and are recognized by this antibody.

Staining Requirements: Use with Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).

Cross-reactivity: Published in rhesus monkey.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated and tested on mouse thymocytes using Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523).

Refer to BestProtocols®:
Protocol B for nuclear intracellular proteins.

Recommended usage: 5 µL (0.06 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Fluorophore Information

PerCP-eFluor® 710 emits at 710 nm and is excited with a 488 nm blue laser.
Recommended filter: 710/50 bandpass (695/40 acceptable alternative).
Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product #00-8222)
• 1-step Fix/Lyse Solution (Product #00-5333)

Excitation: 488 nm
Emission: 710 nm
Laser: Blue
Filtration: 0.2 µm post-manufacturing filtered

Target Information

GATA3 is a zinc finger transcription factor that binds enhancer elements of all four T-cell receptor genes (alpha, beta, gamma, delta).
It is abundantly expressed in T-lymphocytes and regulates T-cell receptor gene activation.
The human GATA3 gene consists of six exons over 17 kb, encoding two zinc fingers highly conserved with GATA1.

For Research Use Only. Not for use in diagnostic procedures.

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