Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), Brilliant Ultra Violet 737, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: ≤ 0.5 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 561
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 805
• Brilliant Violet series (421–786)
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor series (Blue, Red, UV, Violet, Yellow variants)
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright series (436, 600, 645, 702, 780)
• Request custom conjugation available

Product Specific Information

The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed by B cells throughout development except plasma cells. CD19 forms a complex with CD21, CD81, and MHC class II, associating with the BCR to amplify signaling, inducing tyrosine phosphorylation, calcium flux, and proliferation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Validated on mouse splenocytes at ≤ 0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
  Optimal concentration should be titrated per assay.

Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm and is designed for cytometers with a 355 nm UV laser. Ensure instrument compatibility.

When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Staining Buffer (SB-4401) or Brilliant Stain Buffer (00-4409) to minimize non-specific interactions.

• Light Sensitivity: Protect from light.
• Fixation Compatibility: Samples can be stored up to 3 days in the dark at 4°C using IC Fixation Buffer (00-8222) or Fix/Lyse Solution (00-5333).
• Methanol Compatibility: Suitable for short-term methanol fixation (<1 hr).
• Excitation: 355 nm
• Emission: 732 nm
• Laser: Ultraviolet Laser

Trademark Notice: BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains, expressed on all peripheral B cells and some myeloid leukemia cells. It regulates B cell proliferation and differentiation, lowering the threshold for antigen receptor-dependent stimulation.
Mutations cause hypogammaglobulinemia; overexpression leads to B cell hyperactivity.

For Research Use Only. Not for diagnostic or resale without authorization.

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