Thermo Fisher Scientific CD19 Monoclonal Antibody (SJ25C1), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific CD19 Monoclonal Antibody (SJ25C1), Brilliant Ultra Violet 737, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (1.0 µg)/test
Publications: -

Product Specifications

Additional Formats

• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 496
• Brilliant UV 615
• Brilliant UV 805
• Brilliant Violet 605
• Brilliant Violet 650
• eFluor 450
• eFluor 506
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 685
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 590
• NovaFluor Yellow 610
• NovaFluor Yellow 690
• NovaFluor Yellow 755
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

The SJ25C1 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed by B cells during all stages of development except terminally differentiated plasma cells. Follicular dendritic cells also express CD19.

CD19 forms a multimolecular complex with CD21, CD81, Leu13, and MHC class II, associating with the BCR to induce tyrosine phosphorylation, calcium flux, and B cell proliferation. The SJ25C1 and HIB19 clones recognize overlapping epitopes.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells; use at 5 µL (1.0 µg)/test.

Brilliant Ultra Violet™ 737 (BUV737):

• Tandem dye emitting at 732 nm
• For cytometers equipped with 355 nm ultraviolet laser
• Light-sensitive; protect from photo-induced oxidation

Buffer Recommendations:
Use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409) to minimize non-specific polymer interactions.

Fixation Compatibility:

• Store samples in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark.
• Compatible with short-term methanol fixation (<1 hour).

Excitation/Emission: 355 nm / 732 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on 100% of peripheral B cells and on certain myeloid leukemia cells. CD19 acts as a signal-amplifying coreceptor for the B cell receptor (BCR), regulating B cell proliferation and activation.

Mutation of CD19 leads to hypogammaglobulinemia, while overexpression causes B cell hyperactivity. CD19 is a key marker in leukemia phenotype studies and B cell precursor leukemias.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지