Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), Brilliant Ultra Violet 737, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 5 µL (0.125 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661
• Brilliant UV 805
• Brilliant Violet 480
• eFluor 450
• eFluor 506
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), a low-affinity receptor for IgG (50–80 kDa).
CD16 includes two genes: CD16A (FcγRIIIA) expressed in macrophages and NK cells, and CD16B (FcγRIIIB) expressed in neutrophils.
Two polymorphisms, NA-1 and NA-2, affect phagocytic capacity. CD16 binds IgG immune complexes (preferentially IgG1 and IgG3) and mediates ADCC, phagocytosis, and degranulation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and tested on human peripheral blood cells (5 µL/test).

Fluorochrome Information:
Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm, excitable by 355 nm UV laser. Ensure instrument compatibility.

Staining Recommendations:
Use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409) to minimize non-specific polymer interactions.

Light Sensitivity:
Protect vial and stained samples from light.

Fixation:
Samples can be stored up to 3 days at 4°C in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333).
Compatible with short-term methanol fixation (<1 hour).

Excitation: 355 nm
Emission: 732 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD16 encodes an Fc receptor for IgG involved in immune complex clearance, ADCC, and viral infection enhancement.
The gene FCGR3A (chromosome 1) is expressed in NK cells, while FCGR3B is expressed in neutrophils.
Mutations are linked to immunodeficiency 20, lupus, and neonatal neutropenia.
Alternative splicing produces multiple isoforms.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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