Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Brilliant Ultra Violet 737, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 805
• Brilliant Violet 421, 605, 650, 711
• eFluor 450, 506, 615, 660
• FITC
• Functional Grade
• NovaFluor Blue 510, 610-30S, 660-40S, 690
• NovaFluor Yellow 570
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 780
• Request custom conjugation

Product Specific Information

Description:
The N418 monoclonal antibody reacts with mouse CD11c (integrin alphaX), which associates with beta2 integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. It binds to CD54, iC3b, and fibrinogen, playing a role in leukocyte adhesion. N418 binds to CD11c on splenic dendritic cells and precipitates a 150/90 kDa heterodimer.

Applications Reported:
Suitable for flow cytometric analysis.

Applications Tested:
Validated by flow cytometric analysis of mouse splenocytes. Recommended ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best results.

Fluorochrome Information:
Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm and is designed for use with UV (355 nm) lasers. Ensure instrument compatibility.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies (e.g., Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use:

• Super Bright Staining Buffer (Product # SB-4401)
• Brilliant Stain Buffer (Product # 00-4409)

Light Sensitivity:
Protect from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product # 00-8222)
• 1-step Fix/Lyse Solution (Product # 00-5333)
  BUV737 is compatible with short-term methanol fixation (<1 hour).

Excitation/Emission:
355 nm / 732 nm (Ultraviolet Laser)

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD11, together with CD18, forms a heterodimeric adhesion molecule.

• CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils
• CD11b: Found in monocytes, macrophages, NK cells, some B/T cells, granulocytes
• CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and subsets of lymphocytes

For Research Use Only. Not for diagnostic use or resale without authorization.

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