Thermo Fisher Scientific ICAD Monoclonal Antibody (2B10C8)
ICAD 단백질을 표적으로 하는 Thermo Fisher Scientific의 Mouse Monoclonal 항체. Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용에 사용 가능. Protein G로 정제된 액상 형태로, 1 mg/mL 농도. 단기 4℃, 장기 -20℃ 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2B10C8 |
| Immunogen | Purified recombinant fragment of human DFFA (AA: 179–331) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3090969 |
Target Information
ICAD (Inhibitor of Caspase-Activated DNase) is a substrate that regulates nuclear apoptosis by inhibiting caspase-3-activated DNase (CAD). It exists in two isoforms:
- ICAD-L/DFF45 (45 kDa): functional isoform
- ICAD-S/DFF35 (35 kDa): shorter, non-functional isoform
Both isoforms can bind CAD, but only ICAD-L is functionally active. During apoptosis, ICAD is cleaved by caspase-3 at residues 117 and 224, releasing CAD to execute DNA fragmentation. ICAD also acts as a chaperone for CAD folding.
Recent studies suggest ICAD may serve as a potential therapeutic target for restoring normal apoptotic signaling in colon and brain cancer cells. Two alternatively spliced transcript variants encoding distinct isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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