Thermo Fisher Scientific DFFB Monoclonal Antibody (2E6G3)
Thermo Fisher Scientific의 DFFB 단일클론 항체(2E6G3)는 인간 DFFB 단백질을 인식하는 Mouse IgG1 항체로, WB, IHC, Flow Cytometry, ELISA에 적합합니다. Protein G로 정제된 액상 형태이며, 1 mg/mL 농도로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2E6G3 |
| Immunogen | Purified recombinant fragment of human DFFB (AA: 1–289) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3090798 |
Target Information
Caspase-activated DNase (CAD) cleaves chromosomal DNA during apoptosis. Apoptosis, a programmed cell death process, removes toxic or unnecessary cells during mammalian development and can be triggered by various stimuli such as TNF family death factors, UV irradiation, anticancer drugs, and deprivation of survival factors.
During apoptosis, cells and nuclei undergo shrinkage and fragmentation, and chromosomal DNA is degraded into nucleosomal units. CAD, activated by caspases, forms a complex with its inhibitor ICAD. ICAD assists in CAD folding and inhibits its DNase activity. Upon caspase activation, ICAD is cleaved, releasing CAD to enter the nucleus and cleave DNA.
Apoptosis regulation involves the balance between pro- and anti-apoptotic Bcl-2 family proteins, heat shock proteins, and inhibitors of apoptosis proteins (IAPs). Fourteen caspase family members have been identified, seven of which mediate apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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