Thermo Fisher Scientific MDM2 Monoclonal Antibody (IF2)
MDM2 단백질을 인식하는 Thermo Fisher Scientific의 IF2 단클론 항체로, Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합합니다. Human 시료에 반응하며 PBS 기반 용액 형태로 제공됩니다. p53 조절 연구에 유용한 연구용 항체입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–2 µg/mL | View 5 publications |
| Immunohistochemistry (IHC) | – | View 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:500 | View 6 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | 1–5 µg/mL | – |
| Immunocytochemistry (ICC/IF) | 1–5 µg/mL | – |
| Immunoprecipitation (IP) | 1–5 µg/mL | – |
| RNA Immunoprecipitation (RIP) | Assay-dependent | – |
| Miscellaneous PubMed (Misc) | – | View 6 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | IF2 |
| Immunogen | Human MDM2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS (pH 7.2–7.4) with 1 mg/mL BSA, 30% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536824 |
Product Specific Information
This antibody recognizes the ~90 kDa (apparent MW) MDM2 protein and also detects isoforms at approximately 57 and 74/76 kDa.
Originally validated as part of a Thermo Scientific Cellomics High Content Screening Kit, this antibody may require optimization for best performance when used separately.
Target Information
MDM2 is a ubiquitin ligase for p53 and plays a key role in regulating p53 stability. Located on chromosome 12q, MDM2 phosphorylation by Akt at Ser166 and Ser186 enhances interaction with p300, promoting p53 ubiquitination and degradation.
MDM2 overexpression can inhibit p53 transactivation and reduce its tumor suppressor function through E3 ubiquitin ligase activity. It is implicated in apoptosis and cell cycle control and is overexpressed in various cancers including soft tissue carcinomas and breast cancer.
Beyond p53, MDM2 interacts with proteins such as retinoblastoma 1 and ribosomal protein L5, influencing cell cycle and tumorigenesis. More than 40 alternatively spliced transcript variants have been identified in both tumor and normal tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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