Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Super Bright 600, eBioscience
Mouse CD11b 인식 단일클론 항체(M1/70)로 Super Bright 600 형광체 결합. Flow cytometry에 최적화되어 있으며, 405 nm 레이저로 여기되어 600 nm에서 방출. 마우스 및 인간 CD11b 검출 가능, 높은 특이성과 안정성 제공.
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Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Super Bright 600, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 43 publications |
| Immunohistochemistry (PFA fixed) | - | View 5 publications |
| Immunohistochemistry (Frozen) | - | View 3 publications |
| Immunocytochemistry (ICC/IF) | - | View 17 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | View 246 publications |
| Immunoprecipitation (IP) | - | View 1 publication |
| Neutralization (Neu) | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 600 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M1/70 |
| Conjugate | Super Bright™ 600 |
| Excitation / Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637407 |
Additional Available Formats
Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV series, Brilliant Violet series, eFluor series, FITC, PE, PE-Cyanine5, PE-Cyanine7, PerCP-Cyanine5.5, PerCP-eFluor 710, Super Bright series, NovaFluor series 등 다양한 형광체 옵션 제공.
Product Specific Information
The M1/70 monoclonal antibody reacts with mouse CD11b (integrin alphaM, 165–170 kDa). CD11b associates with CD18 to form Mac-1 (αMβ2 integrin) and binds to CD54 (ICAM-1), C3bi, and fibrinogen.
Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes, and mouse B-1 cells. M1/70 also cross-reacts with human CD11b, detecting this antigen on monocytes, granulocytes, and NK cells.
- Reported Applications: Flow cytometric analysis
- Tested Applications: Mouse bone marrow cells; ≤0.125 µg per test
- Laser Compatibility: Violet laser (405 nm), emission at 600 nm
- Filter Recommendation: 610/20 bandpass filter
- Light Sensitivity: Protect from light
- Fixation Compatibility: IC Fixation Buffer or Fix/Lyse Solution (up to 3 days at 2–8°C)
When using multiple Super Bright dyes, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD11b (integrin alpha-M, ITGAM) forms a heterodimer with CD18 (integrin beta-2) known as Mac-1 or CR3.
Expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and microglia.
Functions as a receptor for ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X, mediating cell-cell and cell-matrix interactions.
Used as a microglial marker and involved in monocyte maturation.
Associated with systemic lupus erythematosus and ITGAM-related susceptibility disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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