Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Super Bright 600, eBioscience
마우스 CD11c를 인식하는 N418 단클론 항체로, Super Bright 600 형광염료가 결합된 제품입니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 0.125 µg/test로 사용 가능합니다. 405 nm 레이저로 여기되어 600 nm에서 방출됩니다. 연구용으로만 사용 가능합니다.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Super Bright 600, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 8 publications |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | View 51 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), Super Bright™ 600, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N418 |
| Conjugate | Super Bright™ 600 |
| Excitation / Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2722929 |
Additional Available Formats
Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV series (395–805), Brilliant Violet series (421–711), eFluor series (450–660), FITC, Functional Grade, NovaFluor series, PE, PE-Cyanine series, PerCP-Cyanine5.5, Super Bright series (436–780).
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Product Specific Information
The N418 monoclonal antibody reacts with mouse CD11c (integrin alphaX), which forms a heterodimer with beta2 integrin (CD18). CD11c is expressed by dendritic cells, intestinal intraepithelial lymphocytes, and some activated T cells. It mediates leukocyte adhesion via binding to CD54, iC3b, and fibrinogen.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested on mouse splenocytes at ≤0.125 µg/test (100 µL final volume)
- Recommended Cell Range: 10⁵–10⁸ cells/test
- Optimization: Antibody titration recommended for best performance
Fluorochrome Information:
Super Bright 600 is a tandem dye excited by the violet laser (405 nm) and emits at 600 nm. Recommended filter: 610/20 bandpass. Protect from light due to photo-induced oxidation sensitivity.
Staining Recommendations:
When using multiple Super Bright dyes, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Fixation:
Samples can be stored up to 3 days in the dark at 4°C using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) with minimal effect on brightness and FRET efficiency.
Excitation: 405 nm
Emission: 600 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD11 and CD18 form a heterodimer adhesion molecule complex.
- CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils
- CD11b: Expressed in monocytes, macrophages, NK cells, some B/T cells, granulocytes
- CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and subsets of lymphocytes
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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