Thermo Fisher Scientific CD11c Monoclonal Antibody (N418), Super Bright 600, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV series (395–805), Brilliant Violet series (421–711), eFluor series (450–660), FITC, Functional Grade, NovaFluor series, PE, PE-Cyanine series, PerCP-Cyanine5.5, Super Bright series (436–780).
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Product Specific Information

The N418 monoclonal antibody reacts with mouse CD11c (integrin alphaX), which forms a heterodimer with beta2 integrin (CD18). CD11c is expressed by dendritic cells, intestinal intraepithelial lymphocytes, and some activated T cells. It mediates leukocyte adhesion via binding to CD54, iC3b, and fibrinogen.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes at ≤0.125 µg/test (100 µL final volume)
• Recommended Cell Range: 10⁵–10⁸ cells/test
• Optimization: Antibody titration recommended for best performance

Fluorochrome Information:
Super Bright 600 is a tandem dye excited by the violet laser (405 nm) and emits at 600 nm. Recommended filter: 610/20 bandpass. Protect from light due to photo-induced oxidation sensitivity.

Staining Recommendations:
When using multiple Super Bright dyes, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Fixation:
Samples can be stored up to 3 days in the dark at 4°C using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) with minimal effect on brightness and FRET efficiency.

Excitation: 405 nm
Emission: 600 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD11 and CD18 form a heterodimer adhesion molecule complex.

• CD11a: Expressed in B/T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils
• CD11b: Expressed in monocytes, macrophages, NK cells, some B/T cells, granulocytes
• CD11c: Expressed in monocytes, macrophages, NK cells, some granulocytes, and subsets of lymphocytes

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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