Thermo Fisher Scientific NFIA/NF1A (Nuclear Factor 1A) (Transcription Factor) Monoclonal Antibody (PCRP-NFIA-2C6)
NFIA/NF1A 전사인자에 특이적인 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합합니다. 인간, 마우스, 랫트 시료에 반응하며, 단백질 A/G로 정제된 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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- 4774-MSM2-Px (2개 옵션)
- 판매단위
- pk
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Immunoprecipitation (IP) | 1–2 µg |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2c, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PCRP-NFIA-2C6 |
| Immunogen | Recombinant fragment of human NFIA protein corresponding to protein domain |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
NF-1, also designated CTF, is a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Human NF-1 mRNA analysis has revealed two forms of NF-1 protein arising from alternative splicing of a single gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain and activates transcription through a proline-rich carboxy-terminal transactivation domain. It recognizes and binds the adenovirus type 2 promoter and activates transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element is found in the upstream promoter region of various eukaryotic genes, including Ha-Ras, alpha-globin, HSP70, GRP78, Histone H1, myelin basic protein, and Xenopus laevis vitellogenin gene promoter.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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